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Abstract Background Tropical members of the sponge genus Ircinia possess highly complex microbiomes that perform a broad spectrum of chemical processes that influence host fitness. Despite the pervasive role of microbiomes in Ircinia biology, it is still unknown how they remain in stable association across tropical species. To address this question, we performed a comparative analysis of the microbiomes of 11 Ircinia species using whole-metagenomic shotgun sequencing data to investigate three aspects of bacterial symbiont genomes—the redundancy in metabolic pathways across taxa, the evolution of genes involved in pathogenesis, and the nature of selection acting on genes relevant to secondary metabolism. Results A total of 424 new, high-quality bacterial metagenome-assembled genomes (MAGs) were produced for 10 Caribbean Ircinia species, which were evaluated alongside 113 publicly available MAGs sourced from the Pacific species Ircinia ramosa . Evidence of redundancy was discovered in that the core genes of several primary metabolic pathways could be found in the genomes of multiple bacterial taxa. Across hosts, the metagenomes were depleted in genes relevant to pathogenicity and enriched in eukaryotic-like proteins (ELPs) that likely mimic the hosts’ molecular patterning. Finally, clusters of steroid biosynthesis genes (CSGs), which appear to be under purifying selection and undergo horizontal gene transfer, were found to be a defining feature of Ircinia metagenomes. Conclusions These results illustrate patterns of genome evolution within highly complex microbiomes that illuminate how associations with hosts are maintained. The metabolic redundancy within the microbiomes could help buffer the hosts from changes in the ambient chemical and physical regimes and from fluctuations in the population sizes of the individual microbial strains that make up the microbiome. Additionally, the enrichment of ELPs and depletion of LPS and cellular motility genes provide a model for how alternative strategies to virulence can evolve in microbiomes undergoing mixed-mode transmission that do not ultimately result in higher levels of damage (i.e., pathogenicity) to the host. Our last set of results provides evidence that sterol biosynthesis in Ircinia -associated bacteria is widespread and that these molecules are important for the survival of bacteria in highly complex Ircinia microbiomes. 

This study examined one teacher’s perspectives of effective professional development (PD) four years after attending PD workshops to better understand her uptake and what content, instructional practices, and resources she continues to use, why and how she interprets and implements these practices in her classroom today. We also examined her perspective on the design features of PD that she attributes to her learning. We used a survey and collected video data from the teacher’s classroom, and we also conducted think aloud interviews to better understand her teaching and learning overtime and her perception of her own learning and how it is currently evidenced in her classroom today. Through triangulation of data and analyses, we identified six assertions that capture her perspectives on effective features of PD design as well as providing evidence of her sustained learning four years post PD. 

This study captured middle and high school teachers’ perceptions of what they learned from professional development 3-4 years after participating in one of three NSF funded year-long professional development (PD) projects. We surveyed teachers (n=66) from three different PD projects on the types of content, pedagogy, and resources that they remembered learning and continue to use when teaching mathematics. Results indicate that teachers remember and use many aspects from PD experiences 3-4 years down the road especially those they find relevant to their current teaching position. Most residual learnings from PD also appear to be highly aligned with the goals and intentions of the PD developers and researchers and these learnings have evolved through colleague collaboration and other PD opportunities. 

By-design access to laser wavelength, especially with integrated photonics, is critical to advance quantum sensors, such as optical clocks and quantum-information systems, and open opportunities in optical communication. Semiconductor-laser gain provides exemplary efficiency and integration but merely in developed wavelength bands. Alternatively, nonlinear optics requires control of phase matching, but the principle of nonlinear conversion of a pump laser to a designed wavelength is extensible. We report on laser-wavelength access by versatile customization of optical-parametric oscillation (OPO) with a photonic-crystal ring resonator (PhCR). Leveraging the exquisite control of laser propagation provided by a photonic crystal in a traveling-wave ring resonator, we enable OPO generation across a wavelength range of 1234–2093 nm with a 1550-nm pump and 1016–1110 nm with a 1064-nm pump. Moreover, our platform offers pump-to-sideband conversion efficiency of > 10 % and negligible additive optical-frequency noise across the output range. From laser design to simulation of nonlinear dynamics, we use a Lugiato–Lefever framework that predicts the system characteristics, including bidirectional OPO generation in the PhCR and conversion efficiency in agreement with our observations. Our experiments introduce broadband lasers by design with PhCR OPOs, providing critical functionalities in integrated photonics. 

Sponges are often densely populated by microbes that benefit their hosts through nutrition and bioactive secondary metabolites; however, sponges must simultaneously contend with the toxicity of microbes and thwart microbial overgrowth. Despite these fundamental tenets of sponge biology, the patterns of selection in the host sponges’ genomes that underlie tolerance and control of their microbiomes are still poorly understood. To elucidate these patterns of selection, we performed a population genetic analysis on multiple species of Ircinia from Belize, Florida, and Panama using an F ST -outlier approach on transcriptome-annotated RADseq loci. As part of the analysis, we delimited species boundaries among seven growth forms of Ircinia . Our analyses identified balancing selection in immunity genes that have implications for the hosts’ tolerance of high densities of microbes. Additionally, our results support the hypothesis that each of the seven growth forms constitutes a distinct Ircinia species that is characterized by a unique microbiome. These results illuminate the evolutionary pathways that promote stable associations between host sponges and their microbiomes, and that potentially facilitate ecological divergence among Ircinia species. 

Ookami [3] is a computer technology testbed supported by the United States National Science Foundation. It provides researchers with access to the A64FX processor developed by Fujitsu [17] in collaboration with RIKΞN [35, 37] for the Japanese path to exascale computing, as deployed in Fugaku [36], the fastest computer in the world [34]. By focusing on crucial architectural details, the ARM-based, multi-core, 512-bit SIMD-vector processor with ultrahigh-bandwidth memory promises to retain familiar and successful programming models while achieving very high performance for a wide range of applications. We review relevant technology and system details, and the main body of the paper focuses on initial experiences with the hardware and software ecosystem for micro-benchmarks, mini-apps, and full applications, and starts to answer questions about where such technologies fit into the NSF ecosystem.